A Study On Protein Engineering Essay

1120 Words Sep 2nd, 2015 5 Pages
Protein mutations are often introduced at the DNA level in the corresponding gene, which takes advantage of the central dogma of biology (DNA is transcribed to mRNA; mRNA is translated to proteins. The introduction of mutations in target genes has become trivial with the advances in synthetic biology. Major classes of library construction include (1) random mutagenesis, (2) recombination, (3) semi-rational design, and (4) scanning mutagenesis. Together, these classes of library construction are termed “combinatorial design” and require little to no prior knowledge of the protein, albeit more knowledge can help design libraries more efficiently. The counter technique is termed “rational design” and requires detailed information about the protein for iterative computational mutagenesis. Both design processes have been instrumental in protein engineering, but this section focuses on the main classes of combinatorial design. For a more detailed description of methods for library construction, Packer and Liu recently published an excellent review (Packer and Liu 2015).
Random mutagenesis is a method where substitutions, deletions, or insertions are introduced at random in the target gene, mimicking random mutations introduced during natural evolution, but at an accelerated and tunable rate. Random mutagenesis is particularly appropriate for studying proteins with a lack of structural or functional information. This method can highlight particular “hot spots” in the…

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