Restriction Enzyme Lab Report Essay

2121 Words Mar 19th, 2013 9 Pages
I. Title.
Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis.

II. Authors.
Author:
Partner:
Section: Thursday, 1:10 pm
Date of Experiment: October 25, 2012

III. Introduction. Restriction enzymes (or restriction endonucleases), originally isolated from Haemophilus influenzae in 1970, are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites generally are four to eight base pairs in length. It is thought that, together with enzymes that methylate portions of native DNA, restriction enzymes protect cells from DNA of invading organisms cutting such DNA into pieces, thereby
…show more content…
Fragment |
| | | |(bps)][2] |Length (bps)[3] |
|EcoR1 (E) |
|1 |2.00 |3.61 |4107 |4480 |
|Total bps |4107 |4480 |
|HincII (H) |
|1 |2.55 |3.49 |3105 |3199 |
|2 |4.35 |3.09 | 1243 |1281 |
|Total bps |4348 |4480 |
|PvuII (P) |
|1 |2.00 |3.61 |4107 |4480 |
|Total

Related Documents

Grayce Hampton | Web Therapy | Akagi 183