The Genomic Dna From Drosophila Melanogaster Essay

2283 Words Apr 14th, 2016 null Page
The study of inheritance at the at one of the smallest levels is referred to as molecular genetics and is utilized for many purposes, which include genes, genotyping, sequencing, cloning, etc. (Kelly, 2016). This allows for the ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific section of DNA 9 specific steps had to be conducted and they are as follows: extraction/purification of DNA, amplify the gene utilizing PCR, ligate the product from PCR into plasmid vector, use E. coli to transform plasmid DNA, purify recombinant plasmid DNA, quantify plasmid DNA, conduct restriction analysis of plasmid DNA, conduct DNA sequencing, and lastly analyzed DNA sequence (Garey, Moore, Yoder, Harmon, & Mason, 2016). To begin, a DNA extraction was conducted for Drosophila melanogaster using the method of lysis, which is when the membrane of the cell is compromised and the contents within are released (Brooker, 2009). For the purpose of this experiment sodium dodecyl sulfate, SDS, was utilized. Once all the contents are in one solution, the DNA/RNA were separated using centrifuging to separate fly parts and to phenol to separate the proteins. Resulting in a solution of DNA/RNA, allowing the experiment to proceed (Garey et al., 2016). An important reaction utilized for this…

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